Example sentences for: overhang

How can you use “overhang” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The overhang appears similar to the dorsal blastopore lip in amphibian embryos, and we give it that name.

  • pVL1393-HAX-NFATp(331-921) was made by digesting pBS-KS +-NFATp with SgrA I and filling in the overhang by treatment with Klenow.

  • Another important design aspect that proved critical, according to our preliminary tests, was incorporation of an additional five base G+C rich overhang in the universal primers used for the second round PCR (Figure 2).

  • Studies in Escherichia coli have shown that, although RecA is the principle protein involved in pairing and strand exchange, unrelated proteins, that have a much more restrictive phyletic distribution, can also promote similar reactions in a RecA dependent or RecA-independent manner [ 3 ] . These alternative or additional mediators of homologous recombination include the well-characterized prophage RecT, phage λ Redβ and phage P22 ERF proteins [ 4 5 ] . Similarly, in yeast and vertebrates, the RAD52 protein is involved in the pairing and strand exchange reaction and can promote recombination in a RAD51 (the eukaryotic RecA homolog)-dependent or independent manner [ 6 ] . The RecT protein works in conjunction with the RecE-nuclease [ 7 ] and was initially described in genetic studies on the complemention of mutations in the RecBCD pathway of DNA repair [ 8 9 10 ] . Biochemically, RecT has been shown to bind single-stranded (ss) DNA 3' overhang regions generated by the RecE nuclease, and promote strand exchange between homologous DNA partners by assisting the pairing of complementary single-stranded regions [ 4 10 ] . The reaction catalyzed by the RecT/RecE system is similar to that described for the phage λ exonuclease (exo/Redβ) and the single-strand annealing protein Redβ.

  • 2) and virtually all Bgl I sites (with the exception of sites with CCT overhang) occasionally found in the inserted cDNA sequences were not labelled, as the overhangs created by the restriction digest were not compatible with the overhang of the used oligonucleotides.

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