Example sentences for: osteocalcin

How can you use “osteocalcin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • They accumulated an ill-defined pericellular matrix and the osteoblast-specific protein osteocalcin (Fig.

  • Stro-1 +cells cultured in an osteogenic medium exhibit three markers of differentiated bone: AP; 1,25-dihydroxyvitamin-D 3 -dependent induction of osteocalcin; and a mineralized matrix (hydroxyapatite) [ 36, 37].

  • Rabbit antibodies specific for osteocalcin (1:100 dilution; Peninsula Laboratories, Belmont, Calif.), type I collagen (1:40 dilution; Chemicon, Temecula, Calif.), alkaline phosphatase (1:40 dilution; Sigma), or keyhole limpet hemocyanin (1:40 dilution) were incubated on cell preparations for 45 min at 4°C.

  • This was then amplified by 35 to 50 cycles of the polymerase chain reaction (PCR, GeneAmp RNA PCR kit, N808-0143, Perkin Elmer, Foster City, CA), with primers (Table 1) designed for osteocalcin, type I collagen α1, type II collagen, BMP-2, BMP-4, type IA BMP receptor, 18S ribosomal RNA, or G3PDH (Cat.

  • Much of this information comes from animal cells and embryos, but the addition of BMP2 to cultured postnatal human bone marrow `preosteoblastic' cells in the presence of β-glycerophosphate and ascorbic acid increases the gene message and protein production of AP, osteopontin, bone sialoprotein, osteocalcin, and α-1 collagen [ 33].


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