Example sentences for: incubating

How can you use “incubating” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Non-specific binding sites were blocked by incubating the nitrocellulose for 60 minutes in blocking buffer (25 mM Tris-HCl/Base, 150 mM NaCl, 0.05% Tween-20, and 3% nonfat dry milk).

  • Incubating eggs in normal rabbit sera alone did not result in any reactivity (Fig.

  • cDNA was synthesized from each sample by incubating 1 ug RNA sample with 0.5 mM each dNTP, 0.25 μg/ml random hexamer (Boehringer Mannheim), 10 U RNase inhibitor, and 10 U OmniScript reverse transcriptase in OmniScript RT Buffer (Qiagen).

  • Briefly, TK-1 cells were suspended in culture medium and fluorescence labeled by incubating TK-1 cells at 2 × 10 6cells/ml with 0.02 mg fluorescein diacetate (FDA) (Sigma) at 37°C for 30 min.

  • The titers of the adenoviral stocks were determined by incubating serial dilutions of the stocks at 37°C for 60 min with sub-confluent 293 cells.


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