Example sentences for: immunoprecipitated

How can you use “immunoprecipitated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Blots were then stripped and incubated with anti-RARα antibodies to ensure equal amounts of immunoprecipitated protein in each lane.

  • Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg cdk2 antibody in 300 μl IP buffer [50 mM Tris (pH 7.5), 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, and 1% NP-40] and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.

  • GluR1 was immunoprecipitated with anti-GluR1 antibody and then subjected to in vitro phosphorylation (Figure 4C).

  • Approximately 5 mg of total cell lysates was separated by gel filtration on a 16/60 S300 column (Amersham Pharmacia Biotech), and 1 ml fractions were immunoprecipitated with Pip1p antibodies.

  • The concentration of antigen-specific IgG in standard serum was obtained by measuring the optimal amount of protein immunoprecipitated [ 16 ] , and a standard curve of absorbance against concentration was obtained (Fig.

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