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Example sentences for: img

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Sections from the MPG, the IMG and the sympathetic chain were processed for double-immunofluorescence in order to visualize both MIF and tyrosine hydroxylase, as follows: Sections were exposed to 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Tx-100; 30 min) followed by incubation with anti-MIF (1:200) and anti-tyrosine hydroxylase (sheep polyclonal antibody; 1:1000; Pel-Freez; Rogers, AR) overnight at 4°C.
The following tissues were removed and placed in fixative at least overnight: bladder, ventral lobes of the prostate (in the males), both MPG, the inferior mesenteric ganglia (IMG), the sympathetic chain (L3-L5),
Small intensely fluorescent (SIF) cells, intensely stained with TH, showed little to moderate MIF staining in the IMG (Fig.