Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1 M), d(GAT)TP (25 mM), dCTP (1 mM), Cy3-dCTP (2 mM)(Amersham, Piscataway, NJ) and Superscript RT II (200 units) were added and incubated at room temperature for 10 minutes and then at 42°C for 2 hours.

  • An EGFP probe fragment (~800 base pairs) was prepared by PCR and labeled with 32P-dCTP (Amersham Life Science Inc., Arlington Heights, IL, USA) using a kit (Prime-It RmT, Stratagene, La Jolla, CA, USA).

  • [ 53], including 32P-dCTP (40 mCi/ml; NEN Life Science Products, Boston, MA) in the reaction mixture.

  • Clones were selected at random and used as α 32P-dCTP (Amersham Biosciences Corp, Piscataway, NY, USA) probes on dot blots or Southern blots containing amplicon DNA from the original normal and metastatic cells to verify whether these sequences are actually present in the normal cell DNA sample and missing in the metastatic cell DNA samples.

  • Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.


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