Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Labeled probe was synthesized using a High Prime random priming kit, according to the manufacturer's instructions (Roche Molecular Biochemicals, Indianapolis, IN), from 100 ng of genomic PCR product and 50 μCi [α- 32p]dCTP.

  • 1 M), d(GAT)TP (25 mM), dCTP (1 mM), Cy3-dCTP (2 mM)(Amersham, Piscataway, NJ) and Superscript RT II (200 units) were added and incubated at room temperature for 10 minutes and then at 42°C for 2 hours.

  • The radiolabeled nucleotide [α- 32P]dCTP (3,000 Ci/mmol) and nick translation kits were purchased from Amersham-Pharmacia.

  • In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.

  • Subsequently, a portion of the DNA was labeled with [ 32P]dCTP and used for the next round of site-selection.


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