Example sentences for: amersham

How can you use “amersham” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All transcripts with a different restriction pattern were sequenced (primer sequences available upon request) using the Thermo Sequenase fluorescent labelled primer cycle sequencing kit (Amersham Biosciences) and analysed on an ALF-express automated DNA sequencer (Amersham Biosciences).

  • Western blots were developed using ECL+ (Amersham Pharmacia).

  • pDRF and pDXB were used for the production of internally 32P -labeled RNA substrates by in vitro transcription with the appropriate Megascript kit (Ambion) with addition of 20 μCi [α 32P]UTP (Amersham).

  • The 5'-end of the sagA/pel mRNA was determined by the extension of the 5'-end 32P-labeled oligonucleotides 5'-ACCTTATTTTAAAAATAAAGTTAA-3' following the method of Sambrook [ 45 ] . Oligonucleotides were labeled with [γ- 32P] ATP (10 mCi/mL in aqueous solution) (Amersham, Arlington Heights, IL) and T4 polynucleotide kinase (Gibco BRL Life Technologies, Rockville, MD).

  • The blots were washed three times in phosphate-buffered saline, and the protein complexes were detected with enhanced chemiluminescence detection reagents according to the manufacturer's protocol (Amersham Pharmacia Biotech, Inc, Piscataway, NJ).


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