Example sentences for: agar

How can you use “agar” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sexuals were collected and placed at a ratio of two males to eight females in 16 mm diameter petri dishes holding single leaves of Medicago arborea inserted into 3 ml of 2% agar with 1gL -1of Miracle-Gro (The Scotts Company, OH).

  • Single bacterial colonies from the plasmid library were picked from agar trays into 384-well plates containing media, kanamycin, and 7% glycerol using a Q-bot robotic colony picker (Genetix, Christchurch, UK).

  • To activate the DNA damage checkpoint, Bleomycin was added to cultures (optical density of 0.3) at a concentration of 5 mU/ml for 3 hours [ 28 ] . Thiabendazole (Sigma) was prepared in DMSO as described previously [ 45 ] and added to cooled, YE5S agar prior to pouring plates.

  • DNA fragments containing AQP1 DNA were liberated from pSKAC after restriction and ligated into adenovirus as described previously [ 27 ] . Human embryonic kidney cells (293 cells) were transfected with ligation mixture and individual viruses were isolated from cell lysates by two consecutive rounds of plaque purification using an agar overlay as described previously [ 27 ] . Individual viruses were amplified in 293 cells and purified over cesium step gradient.

  • Prior to use in making inoculum suspensions for biological control assays these strains were grown overnight on BSM agar without kanamycin.


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