Example sentences for: affinity-purified

How can you use “affinity-purified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Pip1p and Pcu1p were detected with affinity-purified rabbit antisera.

  • Antipeptide polymerase β antibodies were affinity-purified from rabbit serum [ 17 ] .

  • Affinity-purified polyclonal antibody (Ab) specific for BMP-5, the blocking peptide for the BMP-5 Ab, and the recombinant human BMP-RIA-Fc chimera were purchased from Research Diagnostics (Flanders, NJ).

  • Following incubation in blocking buffer (10 mM Tris-HCl, pH 7.5, containing 10% non-fat dry milk, 100 mM NaCl, and 0.1% Tween 20), the membranes were probed with an affinity-purified rabbit polyclonal IgG directed against caspase-8 (Caspase-8 p20, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1:200 dilution in blocking buffer, overnight at 4°C.

  • Affinity-purified recombinant Hepatitis B antigen produced in Saccharomyces cerevisae following transformation with the plasmid pCGA7 was used for detection of antibody responses using an ELISA (Aldevron, Fargo, ND) [ 20 ] . The recombinant antigens were diluted in 0.1 M carbonate buffer (pH 9.6) and coated at 1 ug/100 ul per well in 96-well microtitre plates (Dynex Technologies, INC.


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