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Example sentences for: acsf

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In the same slice, tetanic stimulation delivered in control artificial cerebrospinal fluid (ACSF) induced typical γ oscillations, and these γ oscillations were abolished by addition of BMI to the perfusate.
reported that under epileptogenic condition (free Mg 2+in ACSF), γ band oscillations arise from GABAergic depolarizations and that this activity may lead to the generation of ictal discharges [ 18 ] . It has been reported that prolonged periods of γ oscillations are associated with temporal lobe seizures in in vivo rats [ 41 ] . Furthermore, human EEG studies with subdural recording electodes showed that a γ band oscillation could be recorded at the start of typical seizure activity [ 42 ] . In this respect, the hyperthermic γ oscillations may also play a critical role in generation of neuronal epileptiform activity.
Slices were allowed to incubate and recover for at least an hour in room temperature ACSF comprised of the following composition (mM): NaCl 117; KCl 5.4; NaHCO 3 26; MgSO 4 1.3; NaH 2 PO 4 1.2; CaCl 2 2.5; glucose 10, continuously bubbled with 95% O 2 plus 5% CO 2 .
The brain tissue was removed rapidly and placed in iced artificial cerebrospinal fluid (ACSF).
γ oscillations recovered partially by 80 minutes of wash in ACSF.