Example sentences for: acetate

How can you use “acetate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1% gelatin, 1% normal goat serum, 0.3% Triton-X-100 in TBS), placed in primary antibody (1:10 diluted in blocking buffer for all antibodies used) for 2 hours at room temperature, rinsed 3 times for 5 min each in TBS, placed in gold-conjugated secondary antibodies (10 nm gold anti-mouse and 5 nm gold anti-rabbit) for 1 hour, rinsed 3 times in TBS, fixed in 2% glutaraldehyde, rinsed 3 times in TBS, rinsed in water, stained with Reynold=s lead citrate followed by aqueous 2% uranyl acetate, dried on filter paper and viewed using a Philips CM10 transmission electron microscope.

  • Con A, phorbol myristate acetate (PMA), N-acetylcysteine (NAC), brefeldin A and monensin were obtained from Sigma (St.

  • While most cytokine research has focused on cells of the immune system, other cell types produce cytokines including the epithelial cells of the lung [ 28 29 ] , retina [ 30 ] , and intestines [ 31 32 ] . Among the few studies involving the biliary system, it was demonstrated that cultured human intrahepatic duct epithelial cells produce functional IL-8 and MCP-1 (CCL2) [ 14 33 ] . Isolated human intrahepatic epithelial ducts expressed immunoreactive IL-6 when treated with IL-1β or phorbal myristate acetate [ 34 ] . Intracellular TNF-α mRNA and protein was detected in intrahepatic biliary duct cells in tissue sections [ 35 ] , but not in cultured intrahepatic epithelial cells; therefore, there was no clear demonstration that biliary epithelium secretes TNF-α.

  • LPS stimulation of macrophages is known to involve the activation of protein phosphorylation by kinases as well as the activation of nuclear transcription factors such as NF-kappaB [ 10 11 12 13 ] . The activation of protein kinase C (PKC) by diacylglycerol is also a key event in LPS macrophage activation [ 8 ] . In vitro experiments have shown that the secretion of TNF-alpha and IL-1-beta by LPS-stimulated monocytes is dependent upon PKC activation [ 13 14 ] . In this study, we also determined if phorbol myristate acetate (PMA) activation of PKC also enhanced CEES toxicity.

  • Tissues were subsequently rinsed with distilled water, block-stained with saturated aqueous uranyl acetate solution for 1 hour, dehydrated through a graded series of ethanol solutions and embedded in Epon epoxy resin (Ted Pella, Inc.

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