Example sentences for: aat

How can you use “aat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Purification of *AroQ and *Aat Iβfrom P. aeruginosaperiplasm

  • Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.

  • Of course, the spatial location of *Aat Iβ in the periplasm would seem to rule out in vivo function in histidine biosynthesis.

  • 80: C GCT ATC cTT GTT GGT GTT gAT GGT AGG NNN TAA NNN GAA CTG cCC GAG GAA CAT CAG NNN CAA GCG CGT CTG AAT AGG C (regions 2241-2244, 2266-2268, 2272-2274 are randomized, silent mutations are in lower case)

  • AAT is a local alignment software which extended the BLAST algorithm by assigning fixed penalty to long gaps [ 24 ] . To reduce the number of undesirable matches due to interspersed repeats, the DNA sequence is screened for interspersed repeats using the RepeatMasker program (Smit, AFA & Green, P et al http://ftp.genome.washington.edu/RM/RepeatMasker.html).

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