Example sentences for: aaa

How can you use “aaa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The structure of an AAA protomer includes an ATP-binding site located in the cleft between a large α/β N-subdomain and a smaller all-α C-subdomain.

  • However, such primordial di-protomeric AAA polypeptides would have to have been simpler than those present in NSF, ClpA and p97 and other AAA proteins of the current era, for the latter assemble into two-tiered hexameric assemblies, with the axis joining the two protomers in each polypeptide oriented perpendicular to the plane of the hexameric ring [ 14 ] . We cannot, at present, exclude an alternative model for midasin structure in which the hexameric AAA ring assembly is a dimeric two-layered structure formed by two midasin polypeptides arranged laterally with all six odd-numbered protomers in one layer and all six even-numbered protomers in the other.

  • The AAA domain of midasin is evolutionarily related to that of dynein, but it appears to lack a microtubule-binding site.

  • This result supports the view that midasin and dynein evolved from a common AAA ancestral protein that had already developed a subunit structure of six AAA protomers in a single polypeptide.

  • A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.


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