Words similar to aa-binding
Example sentences for: aa-binding
How can you use “aa-binding” in a sentence? Here are some example sentences to help you improve your vocabulary:
Such isozymes have been reported previously [ 11 12 ] . In addition, a 115-kDa APN was detected with Cry1Aa-binding capability (Fig.
Anion-exchange fractions with Cry1Aa-binding ability but without APN enzymatic activity were also size-purifed, and fractions eluting around 175-250 kDa, the MW of BtR175, were collected and concentrated.
[ 21 ] . However, Heliothis virescens (tobacco budworm) APN is only 45% identical and 53% similar to B. mori APN, yet it binds Cry1Aa toxin with high affinity [ 23 ] . Moreover, sequence alignments with APN from Lactobacillus , Streptococcus , Saccharomyces , Arabidopsis , rat, pig, yeast, and human yielded more similarity than H. virescens APN to the putative Cry1Aa-binding region of B. mori APN.
Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
Initially these fractions were predicted to contain the cadherin-like Cry1Aa-binding protein [ 13 14 16 ] . The candidate receptor fractions for APN and cadherin were separately loaded on a size-exclusion column for further purification (Fig.